Surbhi Mundra

5 Questions 11 Answers 0 Followers

Questions related from Surbhi Mundra

How do I determine the kinetic parameters (Km and Vmax) from negative slopes of rate?

Hello, I am trying to calculate the kinetic parameters for my enzyme using fluorescence of the substrate and product. The AUC for the product is not showing much difference, when I freeze and...

07 June 2022 5,697 2 View

How to process fluorescently labeled oligos after HPLC purification?

Hello, I am purifyning fluorescent oligos through HPLC. My desired fraction of the mixture elutes at ~36% acetonitrile in triethylammonium acetate buffer, pH 7.0. I want these fractions to be...

24 September 2020 6,036 2 View

How to process FAM labeled oligos after HPLC purification?

Hello, I am purifying FAM labeled oligo through HPLC. The fractions I collect, are eluted in trietylammonium acetate buffer and acetonitrile mixture. I need to process these oligos, in a way...

21 September 2020 5,874 2 View

Ki determination from the reaction analyzed on HPLC.

Hello, I am trying to measure the kinetic parameters of an enzyme and inhibition kinetics for inhibition reactions, studied through HPLC. Can anyone help me with the path to take for the...

01 January 1970 1,685 4 View

Effect of lyophilization on fluorescently labeled oligos.

I had purified a fluorescent labeled oligo through HPLC. I had collected the peaks from different runs and pooled them and lyophilized. When I run it again on HPLC it is giving multiple peaks....

01 January 1970 5,753 2 View