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Questions related from Suraj Singh
Want to perform NATIVE page for Esterase enzyme. But esterase enzyme substrate is not water soluble its fatty substrate. Synthetic substrates like PNPB are expensive and can’t add to NATIVE gel so...
23 January 2025 797 0 View
I am using a fluorescence substrate Abz-12345-DNP for my enzyme to act on it. Where Abz is fluorophore and Dnp is the quencher and 1234 is my amino acid. I just need to know how we can draw a...
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hello every one, i have protein of size 21Kda which is getting IPTG induced and coming in soluble ...but when I am starting with 500 ml culture and pelleting it down and doing its lysis (50mM tris...
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