Suraj Singh

2 Questions 10 Answers 0 Followers

Questions related from Suraj Singh

How to draw a standard for the fluorescence substrate?

I am using a fluorescence substrate Abz-12345-DNP for my enzyme to act on it. Where Abz is fluorophore and Dnp is the quencher and 1234 is my amino acid. I just need to know how we can draw a...

16 February 2023 9,565 10 View

Protein is not binding to Ni-IDA column?

hello every one, i have protein of size 21Kda which is getting IPTG induced and coming in soluble ...but when I am starting with 500 ml culture and pelleting it down and doing its lysis (50mM tris...

27 September 2021 6,922 8 View