Why we measure the absorbance of light in different wavelength in UV Spectrophotometry? why 750nm in TPC and why 415nm in TFC?

I want to know, what's the cause that we are measuring the absorbance at different wavelength? why not the same? what would be if I used a same wavelength for all the assays related to it.

19 December 2021 3,355 5 View