Supratim Ghatak

5 Questions 11 Answers 0 Followers

Questions related from Supratim Ghatak

Can anyone help me with clinical trial paper on the use of two adrenaline (epinephrine) auto-injector for anaphylaxis?

Guidelines recommend two epinephrine (adrenaline) auto-injectors for the management of anaphylaxis. Can anyone provide me clinical trial papers that showed the benefit of two epinephrine...

10 October 2019 4,728 1 View

When do I apply a drug in colony formation assays?

When you do soft agar colony formation assay, do you treat (with drug/ modulator) cells growing on agar or you pre-treat the cells for a particular incubation period and then seed the treated...

03 March 2015 4,787 5 View

Two drugs have different vehicle/ solvents. Can I use them both in single assay just treating the cell marked for control with two vehicles together?

Its Necessary (I have to tally the effects of these two drugs). Is it Scientifically Correct? same Question for Transfection. Two plasmids have different vector backbones. If I have to tally the...

02 February 2015 10,036 1 View

Lysis Buffer recipe problem?

Our lab is using this lysis buffer for "whole cell lysis" to be used for western blot: Tris-Hcl (pH 8.0) = 50mM NaCl = 150mM NP-40 (Igepal) = 0.1% (v/v) EDTA = 5mM NaF = 50mM Na3VO4 = 1mM PMSF =...

05 May 2013 8,730 11 View

Is a random primer or oligo(dT) better for RT-PCR reaction?

Why?

08 August 2012 7,376 15 View