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Our lab is using this lysis buffer for "whole cell lysis" to be used for western blot: Tris-Hcl (pH 8.0) = 50mM NaCl = 150mM NP-40 (Igepal) = 0.1% (v/v) EDTA = 5mM NaF = 50mM Na3VO4 = 1mM PMSF =...
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Why?
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