Sunita Desairao Patil

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Questions related from Sunita Desairao Patil

Please explain, I have culture supernatent which is NS1 Ag positive and RT-PCR negative. what must be reason for PCR to fail?

I have inoculated serum sample on mosquito cell lines and confirmed virus isolation by NS1Ag ELISA. After that i have done PCR for serotyping but its not working. What must be the reason to have...

04 June 2018 5,765 3 View

Can we perform NS1 antigen detection from cell culture fluid?

I have C6/36 cell lines infected with serum sample. Can i do NS1 antigen detection in supernatant culture fluid? Does it requires purification step?

20 November 2017 7,391 1 View