Sunil Singh

6 Questions 16 Answers 0 Followers

Questions related from Sunil Singh

Rac1 and Q61L Rac1?

Hi everyone, Can i use Q61L Rac1 as a substitute for the GTP bound state of Rac1; as i came across a paper where they have mentioned that the Q61L Rac1 mimics the GTP bound state of Rac1. Kindly...

28 April 2017 1,457 4 View

Protein Sample Purity for Single particle cryo EM?

Hi everyone, I am using a chemical cross-linker to form a complex of three proteins (A-200kDa; B-21kDa; C- 17kDa) for Cryo EM. I get around 20-30% cross linked complex and the size of this complex...

26 April 2017 9,020 5 View

Arginine effects on crystallization?

Hi everyone, Can Arginine inhibit the crystallization process of a protein? I use 500mM Arginine to suppress the aggregation of my protein (30mM HEPES,pH7.5; 150mM NaCl; 5% Glycerol; 500mM...

20 July 2016 7,956 5 View

Suggestions on purification of proteins having membrane binding regions?

Hi everyone, I am trying to express and purify a protein in BL21 (DE3) and arctic express RIL cells, which has a membrane binding region. I am using Triton X100 and CHAPS as detergents for its...

02 May 2016 846 4 View

Any suggestions on how to break protein aggregation?

what chemicals can be added during purification to inhibit protein protein interaction?

03 February 2016 10,108 14 View

What is the best solubility tag for recombinant protein production in E.coli?

I have been trying to purify a human protein in E.coli BL21 cells. I have used GST, MBP, and SlyD. I have to try NusA and Sumo Tag as well. So far, my protein is only soluble with MBP tag....

05 November 2015 4,619 6 View