Sunil Singh

5 Questions 14 Answers 0 Followers

Questions related from Sunil Singh

Rac1 and Q61L Rac1?

Hi everyone, Can i use Q61L Rac1 as a substitute for the GTP bound state of Rac1; as i came across a paper where they have mentioned that the Q61L Rac1 mimics the GTP bound state of Rac1. Kindly...

28 April 2017 1,426 4 View

Protein Sample Purity for Single particle cryo EM?

Hi everyone, I am using a chemical cross-linker to form a complex of three proteins (A-200kDa; B-21kDa; C- 17kDa) for Cryo EM. I get around 20-30% cross linked complex and the size of this complex...

26 April 2017 8,977 5 View

Arginine effects on crystallization?

Hi everyone, Can Arginine inhibit the crystallization process of a protein? I use 500mM Arginine to suppress the aggregation of my protein (30mM HEPES,pH7.5; 150mM NaCl; 5% Glycerol; 500mM...

20 July 2016 7,927 5 View

Suggestions on purification of proteins having membrane binding regions?

Hi everyone, I am trying to express and purify a protein in BL21 (DE3) and arctic express RIL cells, which has a membrane binding region. I am using Triton X100 and CHAPS as detergents for its...

02 May 2016 815 4 View

Any suggestions on how to break protein aggregation?

what chemicals can be added during purification to inhibit protein protein interaction?

03 February 2016 10,063 14 View