Sunil Singh

4 Questions 11 Answers 0 Followers

Questions related from Sunil Singh

Protein Sample Purity for Single particle cryo EM?

Hi everyone, I am using a chemical cross-linker to form a complex of three proteins (A-200kDa; B-21kDa; C- 17kDa) for Cryo EM. I get around 20-30% cross linked complex and the size of this complex...

26 April 2017 8,968 5 View

Arginine effects on crystallization?

Hi everyone, Can Arginine inhibit the crystallization process of a protein? I use 500mM Arginine to suppress the aggregation of my protein (30mM HEPES,pH7.5; 150mM NaCl; 5% Glycerol; 500mM...

20 July 2016 7,916 5 View

Suggestions on purification of proteins having membrane binding regions?

Hi everyone, I am trying to express and purify a protein in BL21 (DE3) and arctic express RIL cells, which has a membrane binding region. I am using Triton X100 and CHAPS as detergents for its...

02 May 2016 806 4 View

Any suggestions on how to break protein aggregation?

what chemicals can be added during purification to inhibit protein protein interaction?

03 February 2016 10,051 14 View