Sunil Kokane

4 Questions 7 Answers 0 Followers

Questions related from Sunil Kokane

Can anyone suggest me how to do conjugation of colloidal Gold nano particle with Polyclonal antibody?

Please give me protocol

17 January 2019 9,095 3 View

Can anybody guide me about nested PCR?

I have a very critical problem related to my above mentioned work. Outer primer specific to this pathogen having amplicon size of 1.8 kb and two pair of inner primer have 1.2 as well 0.8 kb. But...

01 November 2016 1,863 7 View

Any one could help me for optimization of Real Time PCR withTaqman probe ?

Hello, I am working on Real Time PCR withTaqman probe, and used 1 ul DNA (26ng/ul)but i got very late Ct value. how can I optimize for early Ct value? either by increasing amount of DNA or by...

12 August 2016 8,826 6 View

Can we transform plant explant with protein using gene gun ? is there another way for injection of protein in plant?

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06 April 2016 9,347 2 View