Sune Demant

2 Questions 2 Answers 0 Followers

Questions related from Sune Demant

How do you get a nice collagen II staining (immunohistochemistry)?

Dearest hive mind, I have been trying to get a staining going for collagen type II in human specimens (de-calcified). Until now, without great results. My initial approach has been with enzymatic...

11 November 2019 7,533 3 View

At what density do you culture human monocytes when stimulating with mCSF after micro bead purification?

I am currentlyisolating human monocytes on (Ficoll method) and then purifying by means of negative selection with a stnadard micro bead kit. I want to do co culture with fibroblasts, but I am in...

03 March 2015 4,860 6 View