Sundararajan Jayaraman

3 Questions 81 Answers 0 Followers

Questions related from Sundararajan Jayaraman

What is the experience of others in using commercially available primers for 'house keeping' microRNA genes for use in RT-qPCR assays?

Some vendors recommend that when using their primers for house keeping miRNA genes along with universal primer and SYBR Green master mix, it is not necessary to treat RNA preparation with DNAse to...

06 June 2013 2,227 6 View

What are the recommended analysis tools for CAGE (Cap analysis of gene expression) and H3K4me3?

We would like to analyze transcription initiation sites in introns and intergenic regions of interest using bioinformatics approaches.

12 December 2012 8,273 0 View

What is the best method of validating miRNA expression in tissues?

We have compared the global miRNA expression profiling in spleens of diseased and treated mice. What is the most popular method in terms of validating miRNA expression among the following: probe...

11 November 2012 9,959 4 View