Sundar Thiyagarajan

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Questions related from Sundar Thiyagarajan

Why I am not getting accumulated GFP in vacuole for om45 WT GFP yeast strains?

I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...

05 May 2024 6,445 0 View

Why do I observe multiple non-specific bands and the disappearance of the protein marker in my yeast protein Western blot?

I am relatively new to conducting Western blots. I employed the alkaline lysis method to extract whole proteins from yeast. Subsequently, I introduced the Opti Protein Marker (ABM, CAT log NO:...

06 December 2023 1,398 5 View