Should I convert Arbitary unit (AU) to other unit before calculation of FRET efficiency by sensitized emission?

Hlw all, I am confuse with the unit of AU. If I have arbitrary unit for channel 1, 2,  3 and 4 for FRET calculation by sensitization method, should I convert it first to others unit ? E.g. I have...

04 December 2016 8,325 2 View