Sumit Biswas

2 Questions 3 Answers 0 Followers

Questions related from Sumit Biswas

Our purification of protein cleaved from GST tag through a GST sepharose column has been leaky. Any help?

After overnight in-column protease digestion to cleave the GST tag from the protein, the elution always results in a mixture of uncleaved protein, tag, protease and cleaved protein. How do we...

12 December 2014 625 3 View

How can I increase the purity of GST-tagged proteins eluted thru a GST Sepharose column?

How to reduce the additional bands coming after elution?

10 October 2014 5,787 5 View