Sumit Prakash

4 Questions 4 Answers 0 Followers

Questions related from Sumit Prakash

Getting vector sequence instead of cloned fragment sequence with PJET cloning vector ?

Hello all, I'm trying to clone a 0.5 kb fungal DNA fragment, generated from RAPD PCR, into a PJET cloning vector (Thermo Scientific) that has a 98% selection capability. I am verifying the cloned...

27 March 2025 3,672 2 View

Getting vector sequence instead of cloned fragment sequence with PJET cloning vector ?

Hello All, I'm trying to clone a 1.5 kb fungal gene to a PJET cloning vector (Thermo scientific) that has 98% selection capability, and I'm also verifying the clone using the PCR, but every time...

31 December 2024 4,465 2 View

RAPD PCR Band query ?

Hello to all, I am trying to do RAPD PCR with Promega green master mix with parameters 95 : 5min , 95 : 1min, 38 : 1min , 72: 2min with 35 cycles but not getting a very distinct band pattern on...

06 December 2024 6,882 1 View

Getting vector sequence instead of cloned fragment sequence with PJET cloning vector ?

Hello all, I'm trying to clone a 500 kb fungal DNA fragment, generated from RAPD PCR, into a PJET cloning vector (Thermo Scientific) that has a 98% selection capability. I am verifying the cloned...

01 January 1970 1,689 6 View