What is the best purification way to purify protein from Supernatant of Pichia pastoris culture media (basal salts medium)?

My protein has His-tag, Mw 27kDa and pI=10. Which purification step is suitable to purify secretary protein form the Pichia BS culture media, pH 5? Do I need to adjust pH to 7-8 then following...

26 September 2023 7,853 4 View