Sumersing Patil

2 Questions 4 Answers 0 Followers

Questions related from Sumersing Patil

Why does NIH 3T3 lysate yields low protein concentration?

Hi, I am preparing cell lysates using NIH3T3 cells. However I am achieving very less protein concentration. The lysis buffer works well with other cells. Can you share your experience with these...

11 April 2016 2,286 1 View

Anybody experienced with dual luciferase reporter gene assay?

I am establishing a Gli reporter assay in Shh light 2 cells using a Dual luciferase reporter assay system, but I am getting really low luminescence values. However, the same protocol works...

18 June 2014 6,443 3 View