Sumera Sumera

11 Questions 6 Answers 0 Followers

Questions related from Sumera Sumera

Shelf life of laemilli buffer for protein extraction?

Hi good people, I am using laemilli buffer for protein extraction from formalin fixed paraffin embedded (FFPE) tissue sections. In this regard, can I make the buffer one day before protein...

10 October 2017 526 1 View

Protein Extraction from cell lines?

Hi good people, I have got frozen vials of cell lines. I need to know how should I start with this vial for protein extraction? The thawing temperature, the centrifugation speed and all?? Kindly...

23 July 2017 7,684 4 View

Detection of levels of proteins/genes after overexpression and knockdown studies?

Hi, What are the techniques through which I can detect the level of proteins/genes after potentially knocking them down or overexpressing? Can I employ western blotting for overexpression and RT...

12 July 2017 1,642 1 View

Knocking down or over expressing genes in cell lines?

Hi, I need to know the basic techniques for knocking down or over expressing particular genes of interest in human cell lines. Kindly help

07 July 2017 2,972 1 View

Functional Assays?

Hi, What are the functional assays which I can perform to investigate the role of genes after knocking them down or potentially over expressing some genes of interest in cell lines? Kindly help.

06 July 2017 9,080 2 View

How to examine The Cancer Genome Atlas (TCGA) dataset?

Hi, I am looking for the aberrantly expressed S-nitrosylation proteins in glioblastoma multiforme (GBM). I want to examine the TCGA dataset and examine the enzymes responsible for this...

07 May 2017 3,574 3 View

Protein extraction from paraffin embedded tissue blocks?

Hi all, Can i extract proteins from paraffin embedded human tissue blocks? Thankyou

15 March 2017 3,256 1 View

Protein Extraction from U87 MG cell lines?

Hi, Previously, I had done protein extraction from tissue (mice brian). Now I need to extract proteins from cell lines (U87 MG). Can i follow the same protocol? If someone has a protocol or...

23 January 2017 4,591 3 View

What is the best method for Staining and Destaining the gels in SDS-PAGE?

Hi, Someone please explain me the exact solutions which can stain and destain the gels after SDS-PAGE.

22 January 2017 6,236 11 View

Cell lines as control against Glioblastoma cell lines (U87)?

Hi, I am working on Glioblastoma cell lines (U87) and looking for aberrantly expressed glycosylated proteins. which cell lines can I use as control? Can I use normal mice brain tissues as control?

29 December 2016 2,054 3 View

Can I use Lectins for Western Blot Detection of Glycoproteins?

Hi guys, Can i use Lectins instead of antibodies for the detection of Glycoproteins in Western Blotting?

26 August 2016 3,626 4 View