Can anyone help in standardizing the conditions for the amplification of a 120bp DNA fragment, with approx 26 bases 5' overhang in the forward primer?

I am doing an overlapping PCR to ligate 3 DNA fragments. PCR for the first two fragments have worked . I am troubleshooting PCR conditions for the third fragment. This DNA is 120 bp long. The...

04 April 2015 4,216 5 View