Sumanta Barman

4 Questions 5 Answers 0 Followers

Questions related from Sumanta Barman

Is it possible to have similar CDR3 sequence in Naive and Memory B cells?

We know that class switched B cells have similar CDR3 sequence but do Naive B cells have same CDR3 sequence in Memory B cells? If yes why do Naive and Memory B cells have similar CDR3 sequence?...

03 March 2018 5,749 0 View

Could you please tell me how can I work with fastq file to generate counts matrix data as .txt file for R studio?

I have fastq data from Illumina next generation sequencing. I would like to work with this data in R studio to generate heatmap. To generate heat map R studio needs counts matrix data as .txt...

10 October 2016 8,953 1 View

Why do I get band from the sample that I didnot add primer?

Hello, I run a PCR with sample, NC and sample without primer ( I did not add any primer but other reagents are included). I have got same sized band from the sample and the sample without primer....

09 September 2016 9,821 12 View

Does glycoblue have any effect on RNA isolation instead of using glycogen?

Hello.....I have used glycogen for RNA isolation (Trizol protocol) in purpose of next generation sequencing. It worked perfectly though it is difficult to see the small RNA pellet. Then, I tried...

07 July 2016 4,783 5 View