Suman Kumari

3 Questions 12 Answers 0 Followers

Questions related from Suman Kumari

How to separate mitochondrial and cytosolic fractions from tissue?

For my immunoblot analysis, I have to separate out the mitochondrial and cytosolic proteins from tissue. Kindly guide me regarding the separation of cytosolic and mitochondrial proteins. Thanks in...

06 June 2017 8,603 0 View

What is the best way of doing PVDF stripping?

Can anyone suggest me the Chemical preparation and procedure for doing stripping using PVDF membrane? Thank you,

01 January 2017 5,119 7 View

What may be the reason that I am getting gene expression but not protein expression?

Hi,I did my qPCR experiment in kidney tissue for alat2 gene ( mitochondrial alanine aminotransferase) and I got 3-4 fold increase as compared to control, but when I am doing my Western Blotting ,I...

01 January 2016 813 6 View