Sumaira Mehboob

2 Questions 3 Answers 0 Followers

Questions related from Sumaira Mehboob

Why in SDS PAGE sample preparation done in non reductive buffer? especially the refolded protein sample?

the relfoded sample was prepared in SDS containing buffer and hetaed then load on PAGE gel while the same sample was prepared in buffer without SDS but DTT was present and without heating load on...

08 August 2015 6,836 4 View

Which column should I use for the purification of a hydrophilic protein?

I am thinking about using the hydrophobic column but I cannot confirm whether it would be useful or not?

12 December 2013 9,717 3 View