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Questions related from Sumaiah Diab
Hi All I am designing a primer for 3 nucleotide deletions in my gene . These nucleotides are located toward the end of my gene near the tag on my plasmid . I have tried multiple primers and non of...
09 September 2021 1,868 3 View
Hi All I have a technical question regarding the transfection control. For the WT control (where we use the parent plasmid for cloning) Should i make a PCR reaction for it , transformation ,...
30 July 2021 7,959 3 View
HI I am transfecting my DNA into HEK293T cells . The WT cells (which has the plasmid used for cloning) do not show the flag when WB was done , while it was expressing in the mutant i made. Can...
29 July 2021 5,341 3 View
Hi All I am recently doing WB fron transfected cells HEK293(using a plasmid tagged by Myc & DDK) For protein extraction i used RIP buffer and protease inhibitor.The Myc antibody i sued from...
24 July 2021 8,728 6 View
