Has anyone loaded coronary arteries with Fura-2 am using DMT pressure myograph?

I have tried to load rat coronary artery with Fura using DMT pressure myograph set-up, however, the vessel is not loaded. The loading protocol is 4uM Fura, 0.1% Cremophore for 40 min at room...

21 March 2016 6,965 4 View