Can anyone help me with CRISPR CAS9 for knocking down a gene with a point mutation which leads to a single amino acid substitution?

 I am new to this field, and hope someone will able to help me how to go about it.  Do I have to use single gRNA to cut the target gene or two. I will be providing a single strand oligos as a...

17 October 2014 3,594 4 View