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Questions related from Sulgi Lee
I'm a graduate student and I have collected cells from cheekswabs of donors. I have them in bronchial epithelial cell growth media in adherent T25 flasks. The cells look healthy even after three...
10 October 2017 9,115 1 View
Can I pre-amplify a gene of interest using TaqMan gene expression assay for that particular gene from Thermo? If so, do I have to use their TaqMan pre-amp master mix, which is $900 or can I use...
08 May 2017 6,859 2 View
So I'm learning that in order to increase the yield of membrane proteins by biotinylation and strep-magnetic bead pull-down, I must make sure nuclei is intact after lysis, therefore using...
21 June 2016 2,330 2 View
I have tried isolating membrane proteins from glial cells and I have identified fewer and fewer membrane proteins. I cannot pin-point what I am doing wrong. I have attached my protocol (although...
06 June 2016 7,608 4 View
I'm trying to analyze surface membrane proteins from human glial cells. 1. Collect 25 million cells and biotinylate them with 1mg of sulfo-nhs-biotin for 30 min on ice. Buffer is PBS pH 8. 2....
09 May 2016 1,993 5 View
I plan to use hand held homogenizer with a pestle to lyse my cells in as little buffer (8M urea with 10% NP-40) as possible. Will a hand held homogenizer break covalently bound phosphate from...
27 April 2016 7,768 4 View
I'm planning on analyzing phosphopeptides from tissue samples. I was told to extract protein from tissue using either 8M urea buffer with 0.1% NP-40, 1 mM sodium vanadate and protease inhibitor +...
22 April 2016 7,577 9 View
I'm trying to isolate surface membrane protein from a human brain tumor cell line and analyze in mass spec. I'm planning on biotinylating the surface proteins before lysis and performing...
05 April 2016 4,265 1 View
