Suktika Chandra

3 Questions 6 Answers 0 Followers

Questions related from Suktika Chandra

How to improve the quality of genomic DNA extraction from mice tail samples?

Method I use:- a. ~1cm tail tip is cut from the mice b. O/N digestion of tail tissue by Tail Lysis Buffer containing EDTA, Tris, SDS, NaCl and Proteinase K. c. Treatment with 7.5M NH4OAc d. DNA...

09 September 2018 2,550 5 View

Can anybody suggest a positive and a negative control for detecting phospho specific AKT(Ser473) in immunoblots?

I want to check the phospho-AKT level in the lysates of Bone-marrow derived macrophage infected with different kind of viruses. So, i am looking for a good positive and a negative control to run...

12 December 2017 1,612 4 View

Why does PCR to amplify murine Interferon beta, with flanking restriction enzyme sites, from cDNA of Raw264.7 cells does not show any band

I am trying to make a construct of murine Interferon beta (IFNb) fusing with a reporter gene. So, I wanted to amplify the IFNb gene. To do so I designed a primer and the primer had a flanking...

01 January 1970 833 5 View