Sukrit Kapoor

2 Questions 5 Answers 0 Followers

Questions related from Sukrit Kapoor

Why am I not extracting enough RNA from PBMCs and PMNs?

Hi all, I want to extract RNA from PBMCs and PMNs for downstream application but I can't seem to get enough for qPCR. I am using the Monarch Total RNA MiniPrep Kit, and I am using around 2 x...

last month 9,554 7 View

I want to co-culture some bacteria with immune cells, in order to build an in-vitro mastitis model. Any suggestions on how I can do this?

I specifically want to either co-culture PBMCs/Granulocytes with E. coli or S. aureus. After co-culturing, I want to conduct a cytokine analysis after distinct time intervals. Any suggestions or...

18 July 2025 2,478 1 View