Sukhithasri . V

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Questions related from Sukhithasri . V

Can you please recommend an antibody specifically targeting T25 domain (aa 1-224) of Bordetella pertussis adenylate cyclase toxin?

Most of the commercial antibodies available seem to target the T18 domain (aa 225-339) of the adenylate cyclase, or they do not specify which domain. I am in search of an antibody specific to the...

19 December 2023 141 0 View

Can anyone please share a protocol for running a Blue-Native PAGE using BIO-RAD mini gels?

My protein of interest is a bacterial inner membrane associated protein. And I suspect it to be forming an insoluble aggregate in a mutant strain. I read that Blue Native PAGE works better than...

29 August 2020 4,015 2 View