Sukanya Chakraborty

4 Questions 5 Answers 0 Followers

Questions related from Sukanya Chakraborty

Untransformed E. coli growing on amp+ LB plate?

We use DH5 alpha E. coli for transformation and all of our vectors are only amp resistant. We had prepared competent cell and while checking their competency efficiency, there was a lawn of...

17 February 2022 1,388 2 View

Ct value different through out tissues but according to software it is stable. Which one should I believe?

I am trying to identify 2 most stable reference genes to be used in qRT-PCR. I am starting with 10 genes. After real time PCR it is observed that 18s gene shows most stable Ct values through out...

12 June 2017 4,321 3 View

What is the possible reason for getting low RNA concentration and bad 260/280 ratio from plant root sample?

Hi, I am extracting total RNA from plant roots. I am getting low concentration of RNA (~250ng/ul) and the ratio of 260/280 is 1.74. I already have isolated RNA from the same sample previously and...

10 January 2017 4,302 5 View

Self ligation in pJET1.2 Thermo Scientific?

We are using pJET1.2 vector (Thermo CLoneJET PCR Cloning kit) for cloning 1400bp fragment. We have blunted our insert following kit protocol. According to kit self ligated vector should not grow...

12 May 2016 2,997 6 View