Sujit Kumar Verma

2 Questions 2 Answers 0 Followers

Questions related from Sujit Kumar Verma

During Kjeldahl quantification of crude protein determination, how are the Non Protein Nitrgoen (NPN) taken into account?

I want to determine Crude protein concentration of Cocoa husk using Kjeldahl, which estimates the protein in terms of nitrogen content using the equation N X 6.25 (Nitrogen content in amino acid...

05 August 2017 6,004 3 View

Why am I getting 2 bands for the light chain of an antibody on a SDS-PAGE gel?

I have purified a monoclonal antibody from a hybridoma solution, and when I runn it on an SDS-PAGE gel I get 2 bands for the light chain. I know that an antibody is 150 KDa and when we boil it in...

15 May 2014 287 9 View