2 Questions 5 Answers 0 Followers
Questions related from Sujeet Suresh Bhoite
Do I have to perform Dpn1 digestion (of the template strand) immediately after the PCR reaction or can I store the PCR product at -20 and then do the Dpn1 digestion after maybe say 2 or 3 days?
12 December 2015 2,323 7 View
Hi everyone, I am trying to insert a 24 bp fragment into my gene of interest using site directed mutagenesis via whole plasmid PCR. Its not working out, any suggestions about it? My mutagenic...
11 November 2015 8,936 10 View