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Do I have to perform Dpn1 digestion (of the template strand) immediately after the PCR reaction or can I store the PCR product at -20 and then do the Dpn1 digestion after maybe say 2 or 3 days?
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Hi everyone, I am trying to insert a 24 bp fragment into my gene of interest using site directed mutagenesis via whole plasmid PCR. Its not working out, any suggestions about it? My mutagenic...
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