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Questions related from Sujay Ray
I have a protein sample (Vol~10 ml, 120kDa) that I want to concentrate about 3-4 folds. I tried Amicon UltraCentrifuge units with 50kDa molecular wt cutoff filter sets. I get very low recovery....
07 July 2016 6,275 4 View
Total Protein: 6His+MBP+TEV+tagret Protein: pI=6.49, MW=165.315 kDa,Cleaved Protein: target Protein: pI=8.42, MW=121.625 kDadialysis buffer: 20 mM HEPES pH 7.5, 50 mM NaCl, 10% glycerol. (similar...
07 July 2016 5,736 8 View
