Sujay Pal

2 Questions 4 Answers 0 Followers

Questions related from Sujay Pal

Problem in invitro transcription assay : No transcripts have generated.

Hi!! I am trying to do radioactivity based invitro transcription assay using oligo dc tailed template (pGL2CMV) but facing some serious problem. At this moment I am trying to standardize the...

26 March 2017 2,189 2 View

Problem in elution of MBP tagged protein

I am trying to purify an MBP tagged protein from Rosetta-(DE3)-pLysS strain. The gene is cloned into pMAL-c5X vector. The size of MBP is 42 kDa and that of fusion protein is 82kDa. Initially the...

07 March 2016 5,424 12 View