Can anyone suggest how to avoid getting background smears in DGGE (Denaturing gradient gel electrophoresis) gels?

I am getting a background smear besides specifc bands when I run DGGE gels. The PCR products used for DGGE gels were obtained by regular PCR using Taq pol with GC-clamp primers from genomic DNA...

11 November 2012 8,255 10 View