Sujata Ravi

4 Questions 17 Answers 0 Followers

Questions related from Sujata Ravi

Does anyone have a protocol for ICC staining for Pax-7 in human muscle derived stem cells?

I'm using an anti Pax-7 antibody from Santa Cruz Biotech, with fixation in methanol and acetone. The staining is very inconsistent and doesn't appear clearly localised to the nucleus like I've...

27 April 2016 5,451 3 View

Could anyone share a protocol for the isolation of muscle derived stem cells from primary human muscle biopsies?

I am trying to culture the MDSCs from human skeletal muscle and the protocol I'm following is the one published by Eberli et al. in 2009...

06 October 2015 4,831 4 View

Does anyone know a protocol for isolating epithelial cells from skin or buccal mucosa biopsies shorter than the 16 hour protocol with Dispase?

I'm trying to isolate oral epithelial cells, and currently using a modified protocol outlined by Izumi, Marcelo and Feinberg ( 18 hour incubation with Dispase followed by 2.5 hours with...

03 April 2015 9,767 0 View

Could you please recommend a cytoplasmic counterstain for immunocytochemistry?

I am using ICC to stain some nuclear proteins in muscle-derived stem cells. I'm currently using a HRP-conjugated secondary and DAB substrate (From Biogenex) I would like to avoid haematoxylin as a...

01 January 1970 4,790 3 View