Suhnrita Chaudhuri

2 Questions 3 Answers 0 Followers

Questions related from Suhnrita Chaudhuri

What is the recommended ratio of Lenti-vpak packaging kit - packaging plasmids to Lentiviral shRNA constructs used in the Lipofectamine 2000 protocol?

I recently bought Lenti-vpak packaging kit - packaging plasmids from Origene and need to use it for transfection of Lentiviral shRNA constructs into X293T cells. Could you suggest the recommended...

01 January 2016 1,152 1 View

Should I change medium after the DNA-Lipofectamine complexes have been added to X293T cells using the Lipofectamine2000 protocol?

For the Lipofectamine 2000 protocol using X293T cells, how important is it to change the medium after the DNA-Lipofectamine complexes have been added to the cells? I read a protocol from...

10 October 2015 4,905 3 View