Is anti-CD28 antibody required to induce Th17 differentiation in vitro?

Hi, I want to induce Th17 cells from C57BL6 mouse splenocyte CD4+ T cells in vitro, and now I'm preparing for setting up protocol. I planed to induce CD4+ T cells in anti-CD3, anti-CD28, TGFb,...

05 July 2016 7,083 3 View