Sugata Roychowdhury

2 Questions 10 Answers 0 Followers

Questions related from Sugata Roychowdhury

What concentration range should I use to build standard curve for ortho-nitro phenol ?

I would also like to know the linear range of this assay and the detection range? This will be used to measure end point production from beta-gal assay.

03 March 2018 7,495 3 View

How can I separate monomeric protein from its oligomer when both comes out in the flow through?

I am working with HIV-1 gp120 Env protein gp120. Its theoretical pI is 7.91 although it is heavily glycosylated and is being expressed in tobacco plants. I am purifying it sequentially through...

03 March 2015 394 10 View