Sudhakar Pagal

3 Questions 6 Answers 0 Followers

Questions related from Sudhakar Pagal

How can I separate macrophages which have successfully killed internalized bacteria from those that could not do it by flow cytometry?

I need to isolate a tiny fraction of macrophages that could successfully kill the internalized bacteria (for example, M. tuberculosis). I think the best way is FACS, but I need fluorescent dye(s)...

06 June 2019 10,076 4 View

Why am I getting smear on agarose gel begining from the well all the way down, but templates are just 1.5kb length?

Hi, I an joining three fragments - 312bp, 937bp and 313 bp by overlap extension PCR. What I get is bright smears all through the lane, starting from the well down the lane. While the longest...

04 April 2016 9,726 6 View

Is cloning a fragment internal to the target gene is mandatory for single crossover knockout?

I have constructed a Kanamycin cassette with flanking 300 bp forward and reverse sequences (Fwflanking-Kan-Rvflanking) of a target gene which I want to knock-out. For some unknown reasons, the...

04 April 2016 8,067 1 View