Sudeshna Thakur

3 Questions 2 Answers 0 Followers

Questions related from Sudeshna Thakur

How to set up a common annealing temperature for primers in PCR?

I have a primer set. Tm of Forward primer is 56.6 and that of Reverse primer is 63.5. Which common temperature I should use for PCR reaction to gett a good amplification?

14 October 2019 1,432 5 View

Gene amplification in one gene and not other using same cDNA?

I am working with two different genes of whitefly at the same time. Earlier both of the genes were successfully amplified in desired size. All the PCR components and temperature conditions are...

13 July 2019 5,763 4 View

Not able to amplify the gene segment which showed a positive result earlier. Tried three different protocol along with master mix still no result?

Working on insect diuresis gene.

04 April 2019 7,616 2 View