Sucheta De

3 Questions 5 Answers 0 Followers

Questions related from Sucheta De

How to avoid precipitation of protein during dialysis?

I've been trying to dialyze a 1.5ml His-tag protein which has been purified using 250mM imidazole. The dialysis buffer's pH is 7.6 (1X TBS, 0.1% Titon, 5% glycerol) and the pI of my protein is...

20 April 2017 6,301 3 View

What is the concept behind the molar ratio between gene insert and vector in a ligation reaction?

I have 122.7 ng of insert and 227 ng of vector both of which has to be ligated. I'm confused as to how much volume of both vector and gene of interest has to be considered before setting up the...

15 February 2017 6,141 2 View

What might be the reasons for inefficiency of a restriction endonuclease?

I've been trying to digest my gene of interest and vector with BamHI-HF and NdeI in Cutsmart buffer. Both are known to have 100% efficiency in that buffer. BamHI-HF is working fine but NdeI seems...

14 February 2017 9,740 8 View