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I've been trying to dialyze a 1.5ml His-tag protein which has been purified using 250mM imidazole. The dialysis buffer's pH is 7.6 (1X TBS, 0.1% Titon, 5% glycerol) and the pI of my protein is...
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I have 122.7 ng of insert and 227 ng of vector both of which has to be ligated. I'm confused as to how much volume of both vector and gene of interest has to be considered before setting up the...
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I've been trying to digest my gene of interest and vector with BamHI-HF and NdeI in Cutsmart buffer. Both are known to have 100% efficiency in that buffer. BamHI-HF is working fine but NdeI seems...
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