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Questions related from Subhiksha Duraisamy
I've performed OE-PCR to amplify two amplicons (2.8 kb and 1 kb) as a single fragment. I first perform 10 cycles of PCR without the primers to allow fragments with overlap regions of about 30-40...
09 April 2025 7,312 0 View
I'm trying to amplify a specific gene with a primer set, yet each time I get multiple non-specific bands. Sequencing the non-specific bands yields non-specific transcripts. Any inputs on how to...
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