Subhecchha Baidya

2 Questions 4 Answers 0 Followers

Questions related from Subhecchha Baidya

Can I run a purified protein (with His tag) second time through Ni-NTA column?

I have got 4-5 extra bands in SDS gel after running a my eluted protein which was purified through Ni-NTA column. This means impurities are present. My question is, can I again run my protein...

02 February 2020 2,274 2 View

Protein band is not visible in SDS-PAGE?

I have purified my protein (Metallo beta Lactamase, ~28 kDa) by Ni-NTA column. After running it in 15% SDS gel (maintaining standard protocol), I am not getting any band. What could be the...

01 January 2020 1,442 8 View