Subhash Narasimhan

5 Questions 1 Answers 0 Followers

Questions related from Subhash Narasimhan

Can you suggest any study to affirm binding of small peptides interaction with protein which require low concentration of the ligand?

Hello, I want to ask you all for suggestion of any assay of equipment which would require low concentration of ligand to study interaction with protein(K_on and K_off). I understand that ITC...

04 April 2019 2,564 8 View

Why i am unable to reproduce expansion plates?

Hello all, I am currently trying to crystallize a protein which produced thin needles, after standardizing the expansion plates, i improved the dimensions of the needles but, now i am planning to...

10 October 2018 316 0 View

Why i am unable to reproduce expansion plates?

Hello all, I am currently trying to crystallize a protein which produced thin needles, after standardizing the expansion plates, i improved the dimensions of the needles but, now i am planning to...

10 October 2018 2,941 0 View

Protein is colored upon purification?

HI, I am currently purifying a protein(64kDa) with rosetta gammi 2 expression. BUt, i notice a yellow colour in my fractions. It was previously not colored but, recently it is eluting in low...

04 April 2016 6,087 7 View

My protein is degrading after Nickel NTA. Can anyone help?

The cell lysate shows my protein but, after nickel nta it degrades and i get a 54Kda band.But, the size of my protein is 64Kda. Maybe the nterminal is surviving while the c-ter disappears. I have...

03 March 2016 8,070 2 View