Subham Basak

2 Questions 14 Answers 0 Followers

Questions related from Subham Basak

Would you recommend using SYBR green based real time PCR kit for disease diagnostics?

It is seen that most diagnostics kits are Taqman based, now as PCR is quite sensitive, can we settle ourselves with SYBR based kits in a resource-crunch set up?

08 August 2019 9,570 4 View

Is the amount of RNA extracted from normal human serum too little, to give amplification of a marker gene by RT PCR?

I have isolated RNA from 50ul serum and eluted the RNA in 30ul of DEPC- treated water. But when i am preparing 10ul of cDNA from 1ul of the RNA and using it for amplification of a 115bp...

01 January 2019 8,757 3 View