Su Yeong Ma

4 Questions 5 Answers 0 Followers

Questions related from Su Yeong Ma

Does it work as antibiotic resistance if plamid has no RBS??

I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...

12 July 2024 7,821 1 View

Can I insert RBS and His tag in primer to clone interested gene????

I designed primer to clone the target gene with RBS, his tag. I want to confirm below: 1. if Is it okay that extra sequence longer than target gene part?? Targeted gene part is 18bp long But...

11 May 2024 3,065 4 View

Can I insert RBS and His tag in primer to clone interested gene????

I designed primer to clone the target gene with RBS, his tag. I want to confirm below: 1. if Is it okay that extra sequence longer than target gene part?? 2. I designed as RBS located after his...

11 May 2024 7,517 2 View

Would it be fine for further protein expression if the T7 terminator has been inserted as reversely?

I designed a plasmid to express one specific gene and I inserted the T7 terminator after stop codon of gene sequence… and when I checked it was reversed. I am wondering if it will work or not, and...

03 October 2023 3,129 2 View