How many DNA bases and what bases should be inserted between his-tag and enterokinase recognition region in order for enterokinase to work well?

I want to inset a his-tag to a vector. In order to remove this his-tag later, I want to add an enterokinase recognition region, but I am not sure if I need to leave some bases between the his-tag...

21 March 2024 7,154 1 View