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I want to assess the effect of radiation on normal human astrocytes, which are co-cultured with glioblastoma cells. However, a clonogenic assay is not suitable for this since astrocytes do not...
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In the original paper by Skehan et al., and the nature protocol by Vichai et al., they fixated their cells with TCA, without aspirating medium beforehand. In other papers (e.g. Papazisis et al....
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