Steven Welc

3 Questions 6 Answers 0 Followers

Questions related from Steven Welc

What are some in vivo measures of phagocytosis and cell debris clearance?

I am studying muscle injury and repair. In one of my models I observe increased necrotic fibers and debris when looking at HE staining of cross-sections. I would like to evaluate phagocytosis...

23 July 2014 7,847 1 View

How do I prevent the clumping of bone marrow-derived macrophages for flow cytometry?

I have been culturing bone marrow derived macrophages using the protocol published by Zhang X et al. [Curr Protoc Immunol. 2008 November; CHAPTER: Unit–14.1.]. Briefly, 5x10^5 BMCs are cultured...

12 February 2014 903 43 View

Can anyone advise me on using L929 cells to produce culture media for differentiation of macrophages from BMDM?

I am new to macrophage cell culture. Recently I purchased L929 cells from ATCC to condition media for macrophage differentiation. The product sheet that came with the cells has little information...

06 November 2013 4,575 15 View