Steven J Simmons

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Questions related from Steven J Simmons

Does anyone have a protocol for high-affinity nicotinic receptor binding using 3H-epibatidine?

Recently our lab became interested in high-affinity receptor binding using 3H-epibatidine from PerkinElmer. We are thinking of using a single known saturating concentration of 2 nM for each sample...

24 April 2014 5,110 2 View

Anyone know the implications of Imaging Nitrocellulose Blot Upside-Down with Seemingly Intact Signals?

I am wondering the implications of imaging western blots that have been vertically inverted (i.e. lanes still run 1-15 from left to right, but columns run from lightest to heaviest from top to...

14 February 2014 7,884 3 View

Is there a pharmacological method currently available to specifically inhibit phosphorylation of Elk-1 in vivo?

The closest compound I could find is a TAT-DEF-Elk-1 inhibiting peptide that has been used in vitro and is reported in Lavaur et al. 2007 (attached). I am interested in the specific involvement of...

29 December 2013 8,397 2 View

Neurogenesis: Using anti mouse-BrdU primary on mouse tissue.

My lab acquired an interest in examining new cell proliferation in a set of developmental studies in mice via a single i.p. injection of the thymine analog 5-bromodeoxyuridine (BrdU). We have...

12 March 2013 5,553 1 View